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Literature summary extracted from

  • Chiang, C.J.; Chern, J.T.; Wang, J.Y.; Chao, Y.P.
    Facile immobilization of evolved agrobacterium radiobacter carbamoylase with high thermal and oxidative stability (2008), J. Agric. Food Chem., 56, 6348-6354.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.77 biotechnology immobilization of the evolved carbamoylase offers a promising way for the efficient production of D-hydroxyphenylglycine from D-L-hydroxyphenylhydantoin Agrobacterium tumefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.77 the plasmids pET20bl and pET-Chi are used for expression of the protein in Escherichia coli cells Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.77 G75S/V237A mutant, thermal stability 55.6%, wild-type 3.0%, oxidative stability 42.8%, wild-type 5.0% Agrobacterium tumefaciens
3.5.1.77 I286V/F287A mutant, thermal stability 4.5%, wild-type 3.0%, oxidative stability 4.8%, wild-type 5.0% Agrobacterium tumefaciens
3.5.1.77 V237A/C279S mutant, thermal stability 22.4%, wild-type 3.0%, oxidative stability 31.2%, wild-type 5.0% Agrobacterium tumefaciens
3.5.1.77 V40A/G75S/V237A mutant, thermal stability 68.4%, wild-type 3.0%, oxidative stability 80.3%, wild-type 5.0% Agrobacterium tumefaciens
3.5.1.77 V40A/G75S/V237A/I286V/F287A mutant, thermal stability 72.3%, wild-type 3.0%, oxidative stability 83.1%, wild-type 5.0% Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.77 using metal affinity resins, immobilization on chitin beads Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.77 carbamoylase
-
 Agrobacterium tumefaciens
3.5.1.77 N-carbamoyl-D-amino acid amidohydrolase
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.77 70
-
-
 Agrobacterium tumefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.77 35 80 relative enzyme activity 30-25%, 70°C 100% Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.77 7
-
-
 Agrobacterium tumefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.77 6 9 relative enzyme activity 80-30%, pH 7.0 100% Agrobacterium tumefaciens